Date of Graduation
Bachelor of Science
Chemistry & Biochemistry
Microdialysis is a sampling method based on the passive diffusion of solutes across a semi-permeable hollow-fiber membrane that is driven by a concentration gradient. The membrane has a defined molecular weight cutoff, which causes larger molecular solutes such as proteins with molecular weights of approximately 8-80 kDa to have low recoveries. The purpose of this research is to utilize ultrafiltration across the membrane through push-pull and vacuum ultrafiltration methods as a means to increase recovery of large molecular weight analyte. These experiments were carried out using Methyl Orange (MO), Fluorescein Isothiocyanate 4 kDa (FITC-4), and Fluorescein Isothiocyanate 40 kDa (FITC-40). In comparison to conventional microdialysis, using push-pull methods (1 uL/min push with 1, 2, 3, 4, 5, and 8 uL/min pull, respectively) increased MO recovery up to 12%, FITC-4 recovery up to 47%, and FITC-40 recovery up to 37%. In comparison to conventional microdialysis, vacuum ultrafiltration methods (0 uL/min push with 1, 2, 3, 4, 5, and 8 uL/min pull, respectively) increased MO recovery up to 30%, FITC-4 recovery up to 64%, and FITC-40 recovery up to 87%.
Elkins, Michael C., "Increased Microdialysis Recovery of Large Molecular Weight Analytes via Ultrafiltration" (2015). Chemistry & Biochemistry Undergraduate Honors Theses. 11.