Date of Graduation
Bachelor of Science in Chemical Engineering
Stenken, Julie A
Beitle, Robert R.
Committee Member/Second Reader
Clausen, Edgar C.
Many biomolecules including proteolytic enzymes known as matrix metalloproteinases (MMPs) are released when a material, such as a microdialysis probe, is implanted in a living organism. The MMPs cleave structural protein components of the extracellular matrix and play an important role in biological functions such as wound healing.1-3 Many enzymatic substrates for MMPs have fluorescent product mimics, and the mimics can be used as agents to monitor biochemical processes.1 In this study, microdialysis, a minimally invasive sampling technique, was combined with fluorescence imaging to determine the possibility of monitoring microdialysis in real-time, and thus provide a method that could ultimately lead to the ability to monitor localized MMPs.. In vitro studies were conducted with fluorescent dyes IR-820 and Rhodamine B, producing promising results that illustrate combining microdialysis and fluorescence imaging is quite feasible. The IR-820 was unpredictable during in vitro studies; therefore, it was not used in vivo, and Rhodamine B was selected to develop and optimize the combined method due to its stability. Microdialysis probes embedded in agarose phantoms, perfused with Rhodamine B, and imaged via a fluorescent imaging system clearly revealed a diffusion profile developing with time. Increased intensity surrounding the probe during infusion was also visible.
Haynie, J. L. (2014). Fluorescence Imaging of Microdialysis Sampling. Chemical Engineering Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/cheguht/44