Date of Graduation
5-2016
Document Type
Thesis
Degree Name
Bachelor of Science in Chemical Engineering
Degree Level
Undergraduate
Department
Chemical Engineering
Advisor/Mentor
Beitle, Robert
Committee Member/Reader
Hestekin, Christa
Abstract
Current methods of detecting waterborne pathogens involve testing strips which take 24-48 hours to yield results, or require expensive equipment in order to function. In minimalist environments, these two technologies are not always applicable to test water quality. With the emergence of a new method of PCR, named LAMP PCR, it is possible to quickly and accurately detect pathogen DNA in a water sample. In order to scale this technique into a simple device, the aspects of the reaction must be accommodated, and a visual detection method chosen. A handheld device which keeps the isothermal LAMP PCR stable for the desired time was created to meet the former objective, and a fluorescent dye known as EvaGreen was employed for the latter.
Keywords
Biosensor, waterborne pathogens, LAMP PCR, gallium
Citation
Jones, B. C. (2016). Development of a Simple Handheld Biosensor for Waterborne Pathogens. Chemical Engineering Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/cheguht/93
Included in
Biochemical and Biomolecular Engineering Commons, Health Information Technology Commons, Other Chemical Engineering Commons