Date of Graduation
5-2015
Document Type
Thesis
Degree Name
Master of Science in Poultry Science (MS)
Degree Level
Graduate
Department
Poultry Science
Advisor/Mentor
Donoghue, Daniel J.
Committee Member
Slavik, Michael F.
Second Committee Member
Donoghue, Annie M.
Keywords
Biological sciences; Campylobacter jejuni; Chicken; Post-harvest; Pre-harvest; Resorcylic acid
Abstract
Campylobacteriosis is one of the leading foodborne illnesses in United States, and is associated with the consumption of poultry and poultry products. Reducing Campylobacter in these species will reduce the burden of this disease. Unfortunately, most strategies employed to reduce Campylobacter in poultry have either not been successful or produced inconsistent results. One potential control strategy is the use of β-resorcylic acid (BR), a phytophenolic compound classified by the US FDA as "Everything Added to Food in the United States" (EAF 3045) and is therefore deemed safe for consumption. This compounds has antibacterial activity against Salmonella, however, its efficacy to control Campylobacter in poultry has not been evaluated. Preliminary studies in our laboratory demonstrated that BR kills Campylobacter jejuni, in vitro. Therefore, the objective of this study was to evaluate if BR would reduce Campylobacter in chickens. In pre-harvest studies, day of hatch chicks were fed one of five treatments (0, 0.25, 0.5, 1 or 2%BR) in the first trial, whereas a second trial was conducted including two additional doses of 0.75% & 1.5% BR (n=10 chicks/dose). Birds were challenged with mixture of four wild strains of C. jejuni (~ 106 CFU/mL) on day 7 and cecal samples were collected on day 14 and enumerated for Campylobacter. In post-harvest studies, four trials, two each on thigh skin and breast meat, were conducted. Chicken skin or meat samples (2±0.1g) were inoculated with 50µL of C. jejuni (~ 107 CFU/mL). Following 30 min of attachment, samples were dipped into their respective treatment solutions (0, 0.5, 1, 2% BR) for 30 s and suspended 2 min (n=10 samples/dose) and evaluated for reduction in Campylobacter counts. Campylobacter counts were reduced by 1.4 Log CFU/g for the 2% dose in the first pre-harvest trial and by 4.2 or 2.8 Log CFU/g for the 0.5 % & 1% BR doses in the second pre-harvest trial (P
Citation
Wagle, B. (2015). Efficacy of beta-resorcylic Acid to Reduce Campylobacter jejuni in Pre-harvest and Post-harvest Poultry. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/1072
