Date of Graduation
8-2016
Document Type
Dissertation
Degree Name
Doctor of Philosophy in Chemistry (PhD)
Degree Level
Graduate
Department
Chemistry & Biochemistry
Advisor/Mentor
Bill Durham
Committee Member
Roger E. Koeppe
Second Committee Member
Francis Millett
Third Committee Member
Wesley Stites
Keywords
Pure sciences, Biological electron transfer, Cytochrome b5, Metalloproteins, Photoreactive complexes, Ruthenium complexes
Abstract
Photoreactive complexes to study the kinetics of electron transfer of proteins have been in use for a long time. It has always been speculated that complexes bind near the heme or the electron transfer reaction would not occur. But it is unkown exactly how the complex interacts with the protein. The structural, thermodynamic, and kinetic properties of rat liver microsomal cytochrome b5 were investigated when bound to ruthenium dimer complexes. Heteronuclear Single Quantum Coherence studies support a dynamic binding model of a dimer Ru complex bound near the protein’s heme involving residues H39, E44, G42, V61, G62, and H63. The enthalpy of binding ΔH was found to be unfavorable: +200 cal/mol, despite a very favorable equilibrium dissociation constant Kd was found to be 40 µM indicating an entropically controlled process. These insights will help with the design of future photoreactive complexes for the study of electron transfer reactions in metalloproteins.
Citation
Rupar, C. D. (2016). Investigation of the Interaction of Dimeric Ruthenium Complexes with Cytochrome b5. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/1713