Date of Graduation
8-2019
Document Type
Thesis
Degree Name
Master of Science in Biomedical Engineering (MSBME)
Degree Level
Graduate
Department
Biomedical Engineering
Advisor/Mentor
Quinn, Kyle P.
Committee Member
Muldoon, Timothy J.
Second Committee Member
Wolchok, Jeffrey C.
Keywords
skin metabolism; Ischemic skin flap; phasor analysis; skin necrosis
Abstract
Necrotic skin flaps are difficult to predict and treat due to the lack of quantitative biomarkers. Label-free multiphoton microscopy is well suited for non-invasively monitoring skin metabolism through NAD(P)H and other intrinsic fluorophores, and offers immediate future directions for assessing necrosis in the clinic. The objective of this study was to assess whether phasor FLIM could be used to evaluate skin flap status and treatment efficacy in ex vivo skin sections. Phasor maps revealed differences in growth factor treatment and region, but changes in skin flap autofluorescence at 755nm excitation and 460nm emission were not just related to NAD(P)H. A very short lifetime component accumulated in the necrotic distal region of the skin flap, and we partitioned phasor space to detect necrosis, which revealed a sensitivity to IL-10/VEGF and IL-10/HGF gene transfer therapy.
Citation
Toomer, H. R. (2019). Visualizing Ischemic Skin Flap Necrosis Through Phasor Analysis of Autofluorescence Lifetime Images. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/3369
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Bioimaging and Biomedical Optics Commons, Biological Engineering Commons, Molecular, Cellular, and Tissue Engineering Commons, Skin and Connective Tissue Diseases Commons