Date of Graduation

12-2019

Document Type

Thesis

Degree Name

Master of Science in Cell & Molecular Biology (MS)

Degree Level

Graduate

Department

Cell & Molecular Biology

Advisor/Mentor

Trudo, Sabrina P.

Committee Member

Kim, Jae Kyeom

Second Committee Member

Erf, Gisela F.

Keywords

Apiaceous vegetables; Colon cancer; Cruciferous vegetables; Inflammation

Abstract

Previous studies have demonstrated chemo-preventive potential of cruciferous and apiaceous vegetables against colon cancer. Colon inflammation is one condition closely related with colon cancer initiation. Therefore, we wanted to compare if total western diet (TWD) was as pro-inflammatory as died-induced obesity (DIO) and a better dietary model for human conditions, and determine if diet supplementation with cruciferous (broccoli, watercress and cabbage) or apiaceous vegetables (celery and parsnip) could reduce dietary inflammation, and which vegetable was more effective.

Male CBL57/6J mice were fed chow for seven days, on day eight, mice were assigned to one of the following diets: American Institute of Nutrition, 1993 diet for growth, pregnancy and lactation (AIN-93G), DIO, DIO+21% cruciferous (DC), DIO+21% apiaceous (DA), TWD, TWD+21% cruciferous (TC), and TWD+21% apiaceous (TA). On day 84 blood, colon, and liver were collected and stored at -80°C. Cytokine were measured in plasma (ARY006 array), and pro-inflammatory related genes were measured in colon tissue (PAMM-011Z array).

The DIO group did not differ in weight gain compared to any other group. Some cytokines signals in DA were likely to be increased when compared to DIO (G-CSF, IL-5, IL-6 , IL-7, I-TAC, TNF-α, TREM-1; p < 0.005) and when compared to DC (G-CSF, IL-2, IL-5, IL-7, IL-23, IP-10, I-TAC, M-CSF, TARC, TIMP-1, TNF-α; p < 0.005). Bmp2 had a higher mRNA expression in all diets compared to basal diet AIN-93G; p= 0.001. Ccl1 had similar mRNA expression in all diets compared to AIN-93G and DIO; p= 0.001. Ccl19 mRNA was equality expressed in all diets except for DIO; p= 0.000. Ccl4 had a higher mRNA expression in TC diet only when compare with DIO and DC; p= 0.058.

DIO and TWD did not induce inflammation or obesity. Vegetable-supplemented diets induced secretion of some pro-inflammatory cytokines. Cruciferous had a smaller inflammatory response compared to apiaceous vegetables. Although no beneficial effect was seen of vegetables on inflammatory pathways, we do not negate potential chemo-preventive effects by other mechanisms.

Keywords: Colon Cancer, inflammation, cruciferous vegetables and apiaceous vegetables.

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