Date of Graduation

8-2012

Document Type

Thesis

Degree Name

Master of Science in Poultry Science (MS)

Degree Level

Graduate

Department

Poultry Science

Advisor/Mentor

Bottje, Walter G.

Committee Member

Maxwell, Charles V.

Second Committee Member

Wideman, Robert F. Jr.

Third Committee Member

Hargis, Billy M.

Fourth Committee Member

Kong, Byung-Whi

Keywords

Biological sciences; Hepatoma; Oxidative stress; Poultry

Abstract

Bioenergetic mechanisms responsible for ATP production are essential in carrying out maintenance and cell-specific functions. In this study, hepatocytes (liver cells) were used to test both endogenous and exogenous stress on cellular respiration. The secondary lipid peroxide, 4-hydroxynonenal (HNE), was used because it alters bioenergetics by increasing mitochondrial proton leak that attenuates mitochondrial radical production and, therefore, endogenous oxidative stress. The major objective of this study was to demonstrate effects of HNE-induced oxidative stress on avian hepatocyte bioenergetics. Various chemical which help enable the determination of oxygen (O2) consumption linked to ATP synthesis (oligomycin), maximal O2 consumption (FCCP), and proton leak (antimycin a) were used during bioenergetic analysis. Results indicated that both hepatocyte cell lines used exhibited increases in maximal O2 consumption in response to 5 and 10 ìM HNE, whereas 30 ìM HNE enhanced proton leak.

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