Author ORCID Identifier:

https://orcid.org/0000-0003-3709-3696

Date of Graduation

5-2023

Document Type

Thesis

Degree Name

Master of Science in Cell & Molecular Biology (MS)

Degree Level

Graduate

Department

Cell & Molecular Biology

Advisor/Mentor

Samsonraj, Rebekah

Committee Member

wolchok, Jeffrey

Second Committee Member

Harris, Leonard

Third Committee Member

Greene, Nicholas

Fourth Committee Member

Song, Young Hye

Keywords

Genomic Biomarker; GSTT1; Mesenchymal Stem Cell

Abstract

The market demand for hMSCs continues to increase along with the increasing popularity of hMSCs used in the research, medical and industrial fields. This increase has an impact on the need for good quality MSCs. This quality is determined by the ability of MSCs to proliferate and their resistance to senescence. Donor dependent heterogeneity may affect MSC qualities from one individual to another; therefore, a specific biomarker with the ability to identify individuals with desirable hMSC qualities is required. The GSTT1 gene has been found to be associated with hMSC scalability. This gene is deleted in about 38% of the population. Moreover, further validation is needed to identify the relationship of the genotype of the GSTT1 gene with the ability of hMSCs to proliferate and their resistance to senescence. This study aims specifically to look at the relationship between the condition of the GSTT1 gene genotype on the ability of cells to proliferate and on its resistance to senescence. Cell doubling assay and BrDU assay were used to test cell proliferation, while relative telomere length, hTERT expression, and relative expression measurements of genes involved in senescence were used to measure cell resistance to senescence. The results showed that samples without the GSTT1 gene (GSTT1-/-) had better proliferative abilities, and the loss of the GSTT1 gene in the samples also showed cell resistance to senescence.

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