Document Type
Article
Publication Date
11-2023
Keywords
RNA sequencing; small RNA sequencing; RRBS; mitochondrial respiration; oxidative metabolism; epigenetics
Abstract
The central dogma of molecular biology dictates the general flow of molecular information from DNA that leads to a functional cellular outcome. In skeletal muscle fibers, the extent to which global myonuclear transcriptional alterations, accounting for epigenetic and post-transcriptional influences, contribute to an adaptive stress response is not clearly defined. In this investigation, we leveraged an integrated analysis of the myonucleus-specific DNA methylome and transcriptome, as well as myonuclear small RNA profiling to molecularly define the early phase of skeletal muscle fiber hypertrophy. The analysis of myonucleus-specific mature microRNA and other small RNA species provides new directions for exploring muscle adaptation and complemented the methylation and transcriptional information. Our integrated multi-omics interrogation revealed a coordinated myonuclear molecular landscape during muscle loading that coincides with an acute and rapid reduction of oxidative metabolism. This response may favor a biosynthesis-oriented metabolic program that supports rapid hypertrophic growth.
Citation
Ismaeel, A., Thomas, N. T., McCashland, M., Edman, S., Lanner, J. T., Figueiredo, V. C., Fry, C. S., McCarthy, J. J., Wen, Y., Murach, K. A., & Walden, F. v. (2023). Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates With the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice. Function, 5 (1), zqad062. https://doi.org/10.1093/function/zqad062
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
