Document Type

Patent

Publication Date

6-13-2017

Abstract

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine

Department

Biological Sciences; Chemistry & Biochemistry

Patent Number

US9676816

Application Number

US 20160145597 A1

Application Published

5-26-2016

Application Filed

1-21-2014

Assignee

Board of Trustees of the University of Arkansas (Little Rock, AR)

Comments

STATEMENT OF GOVERNMENT RIGHTS The present invention was made with government support under National Institutes of Health (NIH) Grant Number P30 GM103450. The United Stated Government has certain rights to the present invention.

Thallapuranam Krishnaswamy Suresh Kumar, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Srinivas Jayanthi, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Jacqueline Morris, Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR
Alicia D. Brown, Department of Biological Sciences, University of Arkansas, Fayetteville, AR
David S. McNabb, Department of Biological Sciences, University of Arkansas, Fayetteville, AR
Ralph Henry, Department of Biological Sciences, University of Arkansas, Fayetteville, AR

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