Document Type
Patent
Publication Date
9-4-2018
Abstract
Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.
Department
Biological Sciences; Chemical Engineering
Patent Number
US10066232
Application Number
US 20180073030
Application Published
3-15-2018
Application Filed
10-24-2017
Assignee
Board of Trustees of the University of Arkansas (Little Rock, AR); University of Pittsburgh-of the Commonwealth System of Higher Education (Pittsburgh, PA)
Citation
Brune, E. M., Beitle, R., Ataai, M. M., Bartlow, P. R., & Henry, R. (2018). E. Coli Separatome-based Protein Expression and Purification Platform. Patents Granted. Retrieved from https://scholarworks.uark.edu/pat/519
Comments
Ellen M. Brune, Department of Chemical Engineering, University of Arkansas, Fayetteville, AR
Robert Beitle Jr, Department of Chemical Engineering, University of Arkansas, Fayetteville, AR
Ralph Henry, Department of Biological Sciences, University of Arkansas, Fayetteville, AR