Document Type
Article
Publication Date
6-2021
Keywords
leaky gut; FITC-d; pigment; serum; poultry
Abstract
Oral administration of fluorescein isothiocyanate dextran (FITC-d) has been used as an indicator for intestinal permeability in poultry research for several years. Under healthy conditions, tight junctions in the intestinal wall will not allow the 4-6kDa FITC-d to enter the bloodstream. Detection of FITC-d in serum (1-hour post-oral administration of FITC-d) has proven to be a reliable indicator of leaky gut syndrome (increased intestinal inflammation and disruption of tight junctions). Administration of supplementary phytobiotics in feed, particularly products with high beta-carotene levels or other pigments, has resulted in strong serum background fluorescence, which can render this assay unreliable. To account for this increase in background autofluorescence, the FITC-d assay procedure has been modified to accommodate these particular serum samples by including pre-administration serum collection from each treatment group to remove background fluorescence. The modified FITC-d procedure detailed will allow for analysis of intestinal permeability in pigmented serum.
Citation
Vuong, C. N., Mullenix, G. J., Kidd, M. T., Bottje, W. G., Hargis, B. M., & Tellez-Isaias, G. (2021). Research Note: Modified Serum Fluorescein Isothiocyanate Dextran (FITC-d) Assay Procedure to Determine Intestinal Permeability in Poultry Fed Diets High in Natural or Synthetic Pigments. Poultry Science, 100 (6), 101138. https://doi.org/10.1016/j.psj.2021.101138
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
