Date of Graduation

5-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Biological Engineering

Degree Level

Undergraduate

Department

Biological and Agricultural Engineering

Advisor/Mentor

Zhang, Wen

Committee Member/Reader

Haggard, Brian E.

Committee Member/Second Reader

Osborn, G. Scott

Committee Member/Third Reader

Zhang, Wen

Abstract

Harmful Algal Blooms (HABs) are a growing issue worldwide, posing harm to both aquatic ecosystems and drinking water quality. This issue could be potentially mitigated using nanoparticle (NP) treatment, simultaneously removing cyanobacteria and associated cyanotoxins in HABs. This research seeks to discern the effectiveness of using titanium dioxide and iron (III) oxide NP treatment at removing cyanobacteria via flocculation and sedimentation. Each NP at 25 mg/L and 50 mg/L were used to treat suspended culture of Microcystis aeruginosa, the representative cyanobacteria, up to 72 hours. Cell concentration and morphology in the supernatant were measured via a Coulter counter and light microscopy. The decreasing cell concentration in the supernatant showed that both NP can flocculate M. aeruginosa and allow subsequent sedimentation. High concentration NP treatments were more effective than low concentration NP treatments, removing a higher percentage of cells in the same amount of time.

Keywords

M. aeruginosa; Harmful Algal Blooms; Water Quality; Nanoparticle Treatment

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