Evaluating Differences in Optical Properties of Indolent and Aggressive Murine Breast Tumors Using Quantitative Diffuse Reflectance Spectroscopy

Authors

Joel Rodriguez Troncoso, University of Arkansas, FayettevilleFollow
Umme Marium Mim, University of Arkansas, Fayetteville
Jesse D. Ivers, University of Arkansas, FayettevilleFollow
Santosh K. Paidi, Johns Hopkins University
Mason G. Harper, University of Arkansas, FayettevilleFollow
Khue G. Nguyen, University of Arkansas, Fayetteville
Sruthi Ravindranathan, University of Arkansas, FayettevilleFollow
Lisa Rebello, University of Arkansas, Fayetteville
David E. Lee, University of Arkansas, Fayetteville
David A. Zaharoff, University of North Carolina
Ishan Barman, Johns Hopkins University
Narasimhan Rajaram, University of Arkansas, FayettevilleFollow

Document Type

Article

Publication Date

12-2023

Keywords

Spectroscopy; Tumor size; Metastasis

Abstract

We used diffuse reflectance spectroscopy to quantify tissue absorption and scattering-based parameters in similarly sized tumors derived from a panel of four isogenic murine breast cancer cell lines (4T1, 4T07, 168FARN, 67NR) that are each capable of accomplishing different steps of the invasion-metastasis cascade. We found lower tissue scattering, increased hemoglobin concentration, and lower vascular oxygenation in indolent 67NR tumors incapable of metastasis compared with aggressive 4T1 tumors capable of metastasis. Supervised learning statistical approaches were able to accurately differentiate between tumor groups and classify tumors according to their ability to accomplish each step of the invasion-metastasis cascade. We investigated whether the inhibition of metastasis-promoting genes in the highly metastatic 4T1 tumors resulted in measurable optical changes that made these tumors similar to the indolent 67NR tumors. These results demonstrate the potential of diffuse reflectance spectroscopy to noninvasively evaluate tumor biology and discriminate between indolent and aggressive tumors.

Comments

© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.

Citation

Rodriguez Troncoso, J., Mim, U. M., Ivers, J. D., Paidi, S. K., Harper, M. G., Nguyen, K. G., Ravindranathan, S., Rebello, L., Lee, D. E., Zaharoff, D. A., Barman, I., & Rajaram, N. (2023). Evaluating Differences in Optical Properties of Indolent and Aggressive Murine Breast Tumors Using Quantitative Diffuse Reflectance Spectroscopy. Biomedical Optics Express, 14 (12), 6114-6126. https://doi.org/10.1364/BOE.505153