Guar-Based Injectable Hydrogel for Drug Delivery and In Vitro Bone Cell Growth

Authors

Humandra Poudel, University of Arkansas at Little Rock
Ambar R. RanguMagar, Philander Smith University
Pooja Singh, University of Arkansas at Little Rock
Adeolu Oluremi, University of Arkansas at Little Rock
Nawab Ali, University of Arkansas at Little Rock
Fumiya Watanabe, University of Arkansas at Little Rock
Joseph Batta-Mpouma, University of Arkansas, Fayetteville
Jin-Woo Kim, University of Arkansas, Fayetteville
Ahona Ghosh, University of Arkansas at Little Rock
Anindya Ghosh, University of Arkansas at Little Rock

Document Type

Article

Publication Date

9-2023

Keywords

modified natural polymer; injectable hydrogel; drug delivery; drug release kinetics; bone tissue engineering

Abstract

Injectable hydrogels offer numerous advantages in various areas, which include tissue engineering and drug delivery because of their unique properties such as tunability, excellent carrier properties, and biocompatibility. These hydrogels can be administered with minimal invasiveness. In this study, we synthesized an injectable hydrogel by rehydrating lyophilized mixtures of guar adamantane (Guar-ADI) and poly-β-cyclodextrin (p-βCD) in a solution of phosphate-buffered saline (PBS) maintained at pH 7.4. The hydrogel was formed via host-guest interaction between modified guar (Guar-ADI), obtained by reacting guar gum with 1-adamantyl isocyanate (ADI) and p-βCD. Comprehensive characterization of all synthesized materials, including the hydrogel, was performed using nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and rheology. The in vitro drug release study demonstrated the hydrogel’s efficacy in controlled drug delivery, exemplified by the release of bovine serum albumin (BSA) and anastrozole, both of which followed first-order kinetics. Furthermore, the hydrogel displayed excellent biocompatibility and served as an ideal scaffold for promoting the growth of mouse osteoblastic MC3T3 cells as evidenced by the in vitro biocompatibility study.

Citation

Poudel, H.; RanguMagar, A.B.; Singh, P.; Oluremi, A.; Ali, N.; Watanabe, F.; Batta-Mpouma, J.; Kim, J.W.; Ghosh, A.; Ghosh, A. Guar-Based Injectable Hydrogel for Drug Delivery and In Vitro Bone Cell Growth. Bioengineering 2023, 10, 1088. https://doi.org/10.3390/bioengineering10091088

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This work is licensed under a Creative Commons Attribution 4.0 International License.