Genome-Wide Screening of Oxidizing Agent Resistance Genes in Escherichia coli

Authors

Hao Chen, University of Arkansas, FayettevilleFollow
Jessica Wilson, University of Arkansas, FayettevilleFollow
Carson Ercanbrack, University of Arkansas, FayettevilleFollow
Hannah Smith, University of Arkansas, FayettevilleFollow
Qinglei Gan, University of Arkansas, FayettevilleFollow
Chenguang Fan, University of Arkansas, FayettevilleFollow

Document Type

Article

Publication Date

6-2021

Keywords

oxidative stress; hydrogen peroxide; hypochlorite; genome-wide screening; AKSA library

Abstract

The use of oxidizing agents is one of the most favorable approaches to kill bacteria in daily life. However, bacteria have been evolving to survive in the presence of different oxidizing agents. In this study, we aimed to obtain a comprehensive list of genes whose expression can make Escherichia coli cells resistant to different oxidizing agents. For this purpose, we utilized the ASKA library and performed a genome-wide screening of ~4200 E. coli genes. Hydrogen peroxide (H₂O₂) and hypochlorite (HOCl) were tested as representative oxidizing agents in this study. To further validate our screening results, we used different E. coli strains as host cells to express or inactivate selected resistance genes individually. More than 100 genes obtained in this screening were not known to associate with oxidative stress responses before. Thus, this study is expected to facilitate both basic studies on oxidative stress and the development of antibacterial agents.

Citation

Chen, H., Wilson, J., Ercanbrack, C., Smith, H., Gan, Q., & Fan, C. (2021). Genome-Wide Screening of Oxidizing Agent Resistance Genes in Escherichia coli. Antioxidants, 10 (6), 861. https://doi.org/10.3390/antiox10060861

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.