Date of Graduation


Document Type


Degree Name

Bachelor of Science in Chemical Engineering


Chemical Engineering


Sakon, Josh

Committee Member/Reader

Walker, Heather


This thesis covers a two part project: the production methods to create a double collagen binding domain molecule with a growth factor for wound healing applications and the development of a new in-house production method for isolating C1q from bovine blood. The wound healing molecule was created using transformation, sonication, and purification before being tested via electrophoresis SDS page and Western blots to confirm the molecule’s presence. The C1q in-house production method utilizes an ultrafiltration flow cell rather than dialysis at a critical point in the process, allowing for researchers to not only be able to use a single small tank but also greatly reducing the amount of buffer needed for filtration.


wound healing, biomolecular research, plasmid transformation, protein purification, ultrafiltration, C1q