Document Type
Article
Publication Date
3-2022
Keywords
Pseudomonas syringae; type III secretion system; T3SEs; plant-bacteria interactions; necrotic cell death; plant immunity
Abstract
The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) has become a paradigm to investigate plant-bacteria interactions due to its ability to cause disease in the model plant Arabidopsis thaliana. Pst DC3000 uses the type III secretion system to deliver type III secreted effectors (T3SEs) directly into the plant cytoplasm. Pst DC3000 T3SEs contribute to pathogenicity by suppressing plant defense responses and targeting plant’s physiological processes. Although the complete repertoire of effectors encoded in the Pst DC3000 genome have been identified, the specific function for most of them remains to be elucidated. Among those effectors, the mitochondrial-localized T3E HopG1, suppresses plant defense responses and promotes the development of disease symptoms. Here, we show that HopG1 triggers necrotic cell death that enables the growth of adapted and non-adapted pathogens. We further showed that HopG1 interacts with the plant immunity-related protein AtNHR2B and that AtNHR2B attenuates HopG1- virulence functions. These results highlight the importance of HopG1 as a multi-faceted protein and uncover its interplay with AtNHR2B.
Citation
Rodriguez-Puerto, C., Chakraborty, R., Singh, R., Rocha-Loyola, P., & Rojas, C. M. (2022). The Pseudomonas syringae Type III Effector HopG1 Triggers Necrotic Cell Death that is Attenuated by AtNHR2B. Scientific Reports, 12, 5388. https://doi.org/10.1038/s41598-022-09335-1
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
