Document Type
Patent
Publication Date
3-19-2019
Abstract
Methods of gene stacking are described herein. The methods can be used to repeatedly add genes into a chosen locus in a precise manner, which ensures co-segregation of all introduced genes and contributes to the stabilization of gene expression. In addition, methods of removing any additional foreign DNA elements such as selectable markers are provided. Seed stocks or cell lines comprising a gene stacking site, vectors containing an insert flanked by target sites for a site-specific DNA recombinase for use in the methods and kits for carrying out the methods are also provided herein.
Department
Crop, Soil & Environmental Sciences
Patent Number
US10233456
Application Number
US 20160333363 A1
Application Published
11-17-2016
Application Filed
1-30-2015
Assignee
Board of Trustees of the University of Arkansas (Little Rock, AR)
Citation
Srivastava, V. (2019). Method, vectors, cells, seeds and kits for stacking genes into a single genomic site. Patents Granted. Retrieved from https://scholarworks.uark.edu/pat/356
Comments
Vibha Srivastava, Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR