Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens

Authors

Makenly E. Coles, University of Arkansas, FayettevilleFollow
Aaron J. Forga, University of Arkansas, FayettevilleFollow
Roberto Señas-Cuesta, University of Arkansas, FayettevilleFollow
Brittany D. Graham, University of Arkansas, FayettevilleFollow
Callie M. Selby, University of Arkansas, FayettevilleFollow
Alvaro J. Uribe, PromitecFollow
Blanca C. Martinez, PromitecFollow
Jaime A. Angel-Isaza, PromitecFollow
Christine N. Vuong, University of Arkansas, FayettevilleFollow
Xochitl Hernandez-Velasco, National Autonomous University of MexicoFollow
Billy M. Hargis, University of Arkansas, FayettevilleFollow
Guillermo Tellez-Isaias, University of Arkansas, FayettevilleFollow

Document Type

Article

Publication Date

4-2021

Keywords

broiler chickens; essential oils; intestinal permeability; necrotic enteritis; performance

Abstract

The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group (p < 0.05) compared with Group 2. Digested feed supernatant, supplemented with LEO and inoculated with CP, reduced CP burden (p < 0.05). Group 3 also exhibited a significant reduction in FITC-d, IFN-γ and IgA compared with Group 2. However, a significant increase SOD was observed in Group 3 compared with both control groups. Further investigation to compare the effect of LEO and the standard treatment of clostridial NE is required.

Citation

Coles, M. E., Forga, A. J., Señas-Cuesta, R., Graham, B. D., Selby, C. M., Uribe, A. J., Martinez, B. C., Angel-Isaza, J. A., Vuong, C. N., Hernandez-Velasco, X., Hargis, B. M., & Tellez-Isaias, G. (2021). Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens. Animals, 11 (4), 1111. https://doi.org/10.3390/ani11041111

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This work is licensed under a Creative Commons Attribution 4.0 International License.