Blood Plasma Biomarkers for Woody Breast Disease in Commercial Broilers

Authors

Byungwhi C. Kong, University of Arkansas, FayettevilleFollow
Bhuwan Khatri, University of Arkansas, FayettevilleFollow
Seong Kang, University of Arkansas, Fayetteville
Stephanie Shouse, University of Arkansas, Fayetteville
Hakeem J. Kadhim, University of Thi-Qar
Michael Thomas Kidd Jr., University of Arkansas, FayettevilleFollow
Kentu Lassiter, University of Arkansas, FayettevilleFollow
Joseph Hiltz, University of Arkansas, FayettevilleFollow
Barbara Mallmann, University of Arkansas, Fayetteville
Sara Orlowski, University of Arkansas, FayettevilleFollow
Nicholas Anthony, University of Arkansas, Fayetteville
Walter G. Bottje, University of Arkansas, FayettevilleFollow
Wayne Kuenzel, University of Arkansas, FayettevilleFollow
Casey Owens, University of Arkansas, FayettevilleFollow

Document Type

Article

Publication Date

7-2021

Keywords

woody breast; blood plasma; biomarker; proteomics; metabolomics

Abstract

Woody breast (WB) myopathy results in poor muscle quality. The increasing incidence of WB over the last several years indicates a need for improved prediction or early diagnosis. We hypothesized that the use of body fluids, including blood, may be more suitable than breast muscle tissue in developing a minimally invasive diagnostic tool for WB detection. To identify potential early-age-biomarkers that may represent the potential onset of WB, blood samples were collected from 100, 4 wks old commercial male broilers. At 8 wks of age, WB conditions were scored by manual palpation. A total of 32 blood plasma samples (eight for each group of WB and non-WB control birds at two time points, 4 wks and 8 wks) were subjected to shotgun proteomics and untargeted metabolomics to identify differentially abundant plasma proteins and metabolites in WB broilers compared to non-WB control (Con) broilers. From the proteomics assay, 25 and 16 plasma proteins were differentially abundant (p < 0.05) in the 4 and 8 wks old samples, respectively, in WB compared with Con broilers. Of those, FRA10A associated CGG repeat 1 (FRAG10AC1) showed >2-fold higher abundance in WB compared with controls. In the 8 wks old broilers, 4 and 12 plasma proteins displayed higher and lower abundances, respectively, in WB compared with controls. Myosin heavy chain 9 (MYH9) and lipopolysaccharide binding protein (LBP) showed more than 2-fold higher abundances in WB compared with controls, while transferrin (TF) and complement C1s (C1S) showed more than 2-fold lower abundances compared with controls. From the untargeted metabolomics assay, 33 and 19 plasma metabolites were differentially abundant in birds at 4 and 8 wks of age, respectively, in WB compared with controls. In 4 wks old broilers, plasma 3-hydroxybutyric acid (3-HB) and raffinose concentrations showed the highest and lowest fold changes, respectively, in WB compared with controls. The blood plasma 3-HB and raffinose concentrations were confirmed with targeted biochemical assays. Blood biomarkers, such as 3-HB and raffinose, may be suitable candidate targets in the prediction of WB onset at early ages.

Citation

Kong, B. C., Khatri, B., Kang, S., Shouse, S., Kadhim, H. J., Kidd, M. T., Lassiter, K., Hiltz, J., Mallmann, B., Orlowski, S., Anthony, N., Bottje, W. G., Kuenzel, W., & Owens, C. (2021). Blood Plasma Biomarkers for Woody Breast Disease in Commercial Broilers. Frontiers in Physiology, 12, 712694. https://doi.org/10.3389/fphys.2021.712694

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This work is licensed under a Creative Commons Attribution 4.0 International License.