Date of Graduation
5-2016
Document Type
Thesis
Degree Name
Bachelor of Science in Biomedical Engineering
Degree Level
Undergraduate
Department
Biomedical Engineering
Advisor/Mentor
Jensen, Hanna
Committee Member/Reader
Jensen, Morten
Committee Member/Second Reader
Muldoon, Timothy
Committee Member/Third Reader
Puvanakrishnan, Priya
Abstract
The objective of this study was to design citrate-coated gold nanoparticles conjugated with FITC-IgG, a fluorescent antibody, and to qualitatively and quantitatively measure the resulting fluorescent emission. Optical properties of the gold nanoparticles were measured at various stages to provide evidence of successful conjugation. The absorbance spectrum of the citrate gold nanoparticles was compared to that of the reaction mixture containing the gold nanoparticles and the FITC-IgG. A noticeable broadening of the absorption peak was observed at 519 nm indicating a surface modification of the gold nanoparticles. Fluorescence data was obtained with a fluorospectrometer and revealed a significant amount of fluorescent quenching in the reaction mixture as well as the washed mixture containing only fully conjugated molecules. However, the conjugated nanoparticles still emitted fluorescence at 519 nm as shown by the images captured under confocal microscopy. Based on the obtained optical densities of the reaction mixture and the FITC-IgG, the mass of molecules that were conjugated to the nanoparticles was calculated and determined to be approximately 24 FITC-IgG molecules per gold nanoparticle.
Citation
Shah, K. B. (2016). Non-Directional Conjugation of Fluorescent Antibodies to Gold Nanoparticles for Stem Cell Therapy. Biomedical Engineering Undergraduate Honors Theses Retrieved from https://scholarworks.uark.edu/bmeguht/40
Included in
Nanoscience and Nanotechnology Commons, Other Biomedical Engineering and Bioengineering Commons