Document Type
Article
Publication Date
7-2022
Keywords
electrospun membranes; protein purification; hydrophobic interaction chromatography
Abstract
Responsive membranes for hydrophobic interaction chromatography have been fabricated by functionalizing poly(N-vinylcaprolactam) (PVCL) ligands on the substrate of electrospun regenerated cellulose nanofibers. Both static and dynamic binding capacities and product recovery were investigated using bovine serum albumin (BSA) and Immunoglobulin G (IgG) as model proteins. The effects of ligand chain length and chain density on static binding capacity were also studied. A static binding capacity of ~25 mg/mL of membrane volume (MV) can be achieved in optimal ligand grafting conditions. For dynamic binding studies, protein binding capacity increased with protein concentration from 0.1 to 1.0 g/L. Dynamic binding capacity increased from ~8 mg/mL MV at 0.1 g/L BSA to over 30 mg/mL at 1.0 g/L BSA. However, BSA recovery decreased as protein concentration increased from ~98% at 0.1 g/L BSA to 51% at 1 g/L BSA loading concentration. There is a clear trade-off between binding capacity and recovery rate. The electrospun substrate with thicker fibers and more open pore structures is superior to thinner fibrous membrane substrates.
Citation
Chen, S., Wickramasinghe, S. R., & Qian, X. (2022). Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification. Membranes, 12 (7), 714. https://doi.org/10.3390/membranes12070714
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
